Nested Helmholtz coil design for producing homogeneous transient rotating magnetic fields

Citation: Podaru, G., Moore, J., Dani, R. K., Prakash, P., & Chikan, V. (2015). Nested Helmholtz coil design for producing homogeneous transient rotating magnetic fields. Review of Scientific Instruments, 86(3), 6. doi:10.1063/1.4908173Electromagnets that can produce strong rotating magnetic fields at kHz frequencies are potentially very useful to exert rotating force on magnetic nanoparticles as small as few nanometers in size. In this article, the construction of a pulsed high-voltage rotating electromagnet is demonstrated based on a nested Helmholtz coil design. The energy for the coils is provided by two high-voltage discharge capacitors. The triggered spark gaps used in the experiments show sufficient accuracy to achieve the high frequency rotating magnetic field. The measured strength of the rotating magnetic field is 200 mT. This magnetic field is scalable by increasing the number of turns on the coils, by reducing the dimensions of the coils and by increasing the discharge current/voltage of the capacitors. (C) 2015 AIP Publishing LLC

Multilayered broadband antenna for compact embedded implantable medical devices: design and characterization

Design and characterization of a multilayered compact implantable broadband antenna for wireless biotelemetry applications is presented in this paper. The main features of this novel design are miniaturized size, structure that allows integration of electronic circuits of the implantable medical device inside the antenna, and enhanced bandwidth that mitigates possible frequency detuning caused by heterogeneity of biological tissues. Using electromagnetic simulations based on the finite-difference timedomain method, the antenna geometry was optimized to operate in the 401-406 MHz Medical Device Radio communications service band. The proposed design was simulated implanted in a muscle tissue cuboid phantom and implanted in the arm, head, and chest of a high-resolution whole-body anatomical numerical model of an adult human male. The antenna was fabricated using low-temperature co-fired ceramic technology. Measurements validated simulation results for the antenna implanted in muscle tissue cuboid phantom. The proposed compact antenna, with dimensions of 14 mm × 16 mm × 2 mm, presented a −10 dB bandwidth of 103 MHz and 92 MHz for simulations and measurements, respectively. The proposed antenna allows integration of electronic circuit up to 10 mm × 10 mm × 0.5 mm. Specific absorption rate distributions, antenna input power, radiation pattern and the transmission channel between the proposed antenna and a half-wavelength dipole were evaluated

TricycloDNA-modified oligo-2′-deoxyribonucleotides reduce scavenger receptor B1 mRNA in hepatic and extra-hepatic tissues—a comparative study of oligonucleotide length, design and chemistry

We report the evaluation of 20-, 18-, 16- and 14-mer phosphorothioate (PS)-modified tricycloDNA (tcDNA) gapmer antisense oligonucleotides (ASOs) in Tm, cell culture and animal experiments and compare them to their gap-matched 20-mer 2′-O-methoxyethyl (MOE) and 14-mer 2′,4′-constrained ethyl (cEt) counterparts. The sequence-matched 20-mer tcDNA and MOE ASOs showed similar Tm and activity in cell culture under free-uptake and cationic lipid-mediated transfection conditions, while the 18-, 16- and 14-mer tcDNA ASOs were moderate to significantly less active. These observations were recapitulated in the animal experiments where the 20-mer tcDNA ASO formulated in saline showed excellent activity (ED50 3.9 mg/kg) for reducing SR-B1 mRNA in liver. The tcDNA 20-mer ASO also showed better activity than the MOE 20-mer in several extra-hepatic tissues such as kidney, heart, diaphragm, lung, fat, gastrocnemius and quadriceps. Interestingly, the 14-mer cEt ASO showed the best activity in the animal experiments despite significantly lower Tm and 5-fold reduced activity in cell culture relative to the 20-mer tcDNA and MOE-modified ASOs. Our experiments establish tcDNA as a useful modification for antisense therapeutics and highlight the role of chemical modifications in influencing ASO pharmacology and pharmacokinetic properties in animal